Establishment of a transplantable human myeloid BALB/c nude mouse model / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 596-600, 2005.
Article
in English
| WPRIM
| ID: wpr-356507
ABSTRACT
To establish a mouse model bearing transplantable human chronic myeloid leukemia for hematopoietic stem cell transplantation to treat leukemia, 4 - 5-week-old female BALB/c nude mice were given cyclophosphamide 2 mg/mouse at day -2, -1, and then the human chronic myeloid leukemia K562 cells were engrafted into the mice at day 0 by injection via tail vein or peritoneal cavity. PB and BM cells were collected, the CD45, CD13, and CD33 antigens were delected by using FCM, the bcr/abl fusion gene mRNA was examined by RT-PCR. The results showed that transplantable leukemic mice could be yielded from 4 - 5-week-old nude mice either by injection through tail vein or peritoneal cavity when the total number of inoculated tumor cells was more than 2 x 10(5) per mouse, whether being pretreated with 2 mg CTX/mouse or not. The transplanted mice could survive 30 - 60 day with leukemia. In conclusion, the mouse model bearing leukemia can be established by inoculation 2 x 10(5) K562 cells into immunodeficient BALB/c nude mice.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Transplantation, Heterologous
/
Blood
/
RNA, Messenger
/
Antigens, Differentiation, Myelomonocytic
/
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
/
Leukemia, Experimental
/
Antigens, CD
/
Gene Expression Regulation, Leukemic
Limits:
Animals
/
Female
/
Humans
Language:
English
Journal:
Journal of Experimental Hematology
Year:
2005
Type:
Article
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