Experimental study on apoptosis in leukemia cells induced by econazole / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 391-393, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-356552
ABSTRACT
To investigate apoptosis of mouse leukemia cell (WEHI-3) induced by econazole and its mechanism, apoptosis induced by econazole was examined by flow cytometry, while free calcium ([Ca(2+)]i) was determined by Fura-2 fluorescein load technique. The protein was isolated from endoplasmic reticulum of WEHI-3 cells, and then the expression of caspase-12 and caspase-7 was evaluated by Western blot. The results showed that WEHI-3 exhibited typical change of apoptosis when it was treated by econazole, [Ca(2+)]i was significantly higher in comparison with the control. The expression of caspase-12 and caspase-7 enhanced as the econazole concentration increased. In conclusion, econazole can induce WEHI-3 cell apoptosis and the caspase-12 plays a key role in this process.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Leukemia
/
Blotting, Western
/
Calcium
/
Apoptosis
/
Cell Line, Tumor
/
Dose-Response Relationship, Drug
/
Econazole
/
Caspase 7
Limits:
Animals
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2005
Type:
Article
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