Role of Bcl-xL in the cathepsin D-associated apoptosis of K562 cells / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 379-382, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-356555
ABSTRACT
The purpose of study was to explore the possible functions of Bcl-xL in the glucosamine sulfate-induced apoptosis of chronic myeloid leukemia K562 cells. Light microscopy and Wright-Giemsa staining were used to investigate the morphologic evidences for apoptosis of K562 cells induced by glucosamine sulfate (GS); immunofluorescence was used to observe the translocation of cathepsin D and cytochrome C during the apoptosis; Western blot was performed to detect the expression of Bcl-xL, Bid, Bax in K562 cells treated by GS. The results showed that many vacuoles were observed in the cytoplasma of the K562 cells treated by GS; fluorescent signals of cathepsin D and cytochrome were fransformed from granules to disperse form by using immunofluorescence; the expression of Bcl-xL was found down-regulated in K562 cells treated by GS, but not in the cells pre-treated with pepstatin A; the significant changes were not detected in expression of Bax and Bid protein before or after apoptosis. It is concluded that Bcl-xL protein may mediate relationship between cathepsin D and mitochondia pathway, Cathepsin D may play an important role in the GS inducing apoptosis of K562 cells through downregulation of Bcl-xL expression.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Physiology
/
Blotting, Western
/
Fluorescent Antibody Technique
/
Cathepsin D
/
Apoptosis
/
K562 Cells
/
Cytochromes c
/
Bcl-2-Associated X Protein
/
Bcl-X Protein
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2005
Type:
Article
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