Establishment of cytokine-independent human myelodysplastic cell line and its characteristics / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 298-303, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-356573
ABSTRACT
This study was aimed to establish a cytokine-independent human myelodysplastic cells line from bone marrow of a patient with MDS-CMML. This cell line was incubated in mixed culture of RPMI 1640 and DMEM with 15% bovine serum, but without cytokines; its biological characteristics were identified by morphology, surface marker profiles, cell proliferation, differentiation and apoptosis. The results showed that the established cell line could not depend on cytokines for long-term survival and growth, and could differentiate into colony-forming unit-macrophage, colony-forming unit-megakaryocyte. In conclusion, a cytokine-independent human myelodysplastic syndrome cell line, named MDS-JSN04 (MDS Nanjing Jiansu 04), was established. Its partial biological characteristics were identified and clarified.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Time Factors
/
Myelodysplastic Syndromes
/
Bone Marrow Cells
/
Microscopy, Electron, Scanning
/
HLA-DR Antigens
/
Cell Differentiation
/
Cell Line
/
Cytokines
Type of study:
Prognostic study
Limits:
Humans
/
Male
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2005
Type:
Article
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