Expansion in vitro and cytotoxicity of dendritic cells from patients with chronic myeloid leukemia / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 198-204, 2005.
Article
in English
| WPRIM
| ID: wpr-356593
ABSTRACT
The study was aimed to investigate the extensive amplification and the cytotoxicity of dendritic cells (DC) derived from chronic myeloid leukemia cells. DC were cultured in two steps:
firstly, extensive amplification in primary culture of CD34(+) or mononuclear cells isolated from CML patients' bone marrow and peripheral blood with rhFlt3-L and rhTPO for 7 days; secondly, inducing culture of DC with rhGM-CSF, rhTNF and rhIL-4 for 14 days. A system inducing DC directly were established for comparison. DC were identified by immunophenotype with flow cytometry, chromosome analysis by displaying G banding and electric microscopy analysis. The function of stimulating T cells proliferation and cytotoxicity of CML cells were confirmed through MTT assay. The results showed that after first extensive amplification in primary culture with rhFlt3-L and rhTPO for 7 days, CD34(+) cells had a total cell number with (77 +/- 5) fold expansion, and DC were (39 +/- 8)% of total cell respectively after induction culture of DC with rhGM-CSF, rhTNF and rhIL-4 for 14 days. Both the amplification of cell number and yield of DC were higher than the system without extensively culture (P < 0.01). Such DC could stimulate T cells to proliferate and kill leukemia cells finally. In conclusion, two-step culture method can obviously improve the cell number of DC required, that is better than inducing them directly. DC derived from CML cells induce the generation of anti-leukemia immunization.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Dendritic Cells
/
T-Lymphocytes, Cytotoxic
/
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
/
Cells, Cultured
/
Immunophenotyping
/
Antigens, CD34
/
Cytotoxicity, Immunologic
/
Cell Proliferation
/
Allergy and Immunology
Limits:
Adolescent
/
Adult
/
Aged
/
Child
/
Female
/
Humans
/
Male
Language:
English
Journal:
Journal of Experimental Hematology
Year:
2005
Type:
Article
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