Study on the B cell linear epitopes of rabies virus CVS-11 nucleoprotein / 病毒学报
Chinese Journal of Virology
;
(6): 253-256, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-356607
ABSTRACT
To study the B cell linear epitopes of rabies virus CVS-11 nucleoprotein, peptides were synthesized according to the amino acid sequences of B cell linear epitopes. Linear epitopes predicted by bioinformatics analysis were evaluated with immunological techniques. Indirect enzyme-linked immunosorbent assay showed that titers of antibodies to peptides (355-369 and 385-400 residues of rabies virus CVS-11 nucleoprotein) were above 112 800 in mouse sera. The antibodies recognized denatured rabies virus CVS-11 nucleoprotein in Western blot analysis. Purified anti-peptide antibodies recognized natural rabies virus CVS-11 nucleoprotein in BHK-21 cells in indirect fluorescent antibody test. The 355-369 and 385-400 residues of rabies virus CVS-11 nucleoprotein were validated as B cell linear epitopes.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Rabies
/
Rabies virus
/
Virology
/
Molecular Sequence Data
/
Chemistry
/
Amino Acid Sequence
/
Epitope Mapping
/
Epitopes, B-Lymphocyte
/
Allergy and Immunology
/
Genetics
Limits:
Animals
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2014
Type:
Article
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