Severe fever with thrombocytopenia syndrome virus nucleoprotein specifically binds to 60kD SSA/Ro protein in host cells / 病毒学报
Chinese Journal of Virology
;
(6): 233-237, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-356610
ABSTRACT
This study aims to investigate whether the nucleoprotein (NP) of severe fever with thrombocytopenia syndrome virus (SFTSV) can impact the cellular immunity of host cells. Gene segments that encode the NP and non-structural protein (NSs) of SFTSV were inserted into eukaryotic expression vector VR1012. Host proteins that interact with NP and affect immunity were identified with co-immunoprecipitation (IP), SDS-PAGE, mass spectrometry (MS), and Western blot. Co-localization of NP and the identified host proteins was confirmed by confocal microscopy. A 60kD SSA/Ro, a protein related to immunity, interacted with NP, as found by IP and MS. Confocal microscopy showed that NP and SSA/Ro were co-localized in cytoplasm. These results indicated that SFTSV NP may specifically bind to 60kD SSA/Ro and cause a series of immune responses and clinical symptoms.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Protein Binding
/
Ribonucleoproteins
/
Viral Proteins
/
Virology
/
Phlebovirus
/
Bunyaviridae Infections
/
HEK293 Cells
/
Genetics
/
Metabolism
/
Nucleoproteins
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2014
Type:
Article
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