IL15 DNA adjuvant enhances cellular and humoral immune responses induced by DNA and adenoviral vectors encoding HIV-1 subtype B gp160 gene / 病毒学报
Chinese Journal of Virology
;
(6): 62-65, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-356637
ABSTRACT
To enhance the immunogenicity of DNA and adenoviral vector vaccines expressing HIV-1 subtype B gp160, human interleukin 15 (hIL15) DNA adjuvant (pVR-hIL15) was constructed. BALB/c mice received DNA prime/protein boost immunization with pVR-HIVgp160/Ad5-HIVgp160 alone or combined with pVR-hIL15. Cellular and humoral immune responses were evaluated by IFN-gamma enzyme-linked immunosorbent spot assay and enzyme-linked immunosorbent assay, respectively. Compared with those immunized with vaccines alone, the mice immunized with vaccines combined with pVR-hIL15 had significantly increased specific cellular response and antibody titer (P < 0.05). It suggests that the IL15 DNA adjuvant can enhance the immune responses induced by prime-boost regimen using DNA and adenoviral vector encoding HIV-1 subtype B gp160.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
HIV Envelope Protein gp41
/
HIV Envelope Protein gp120
/
Adenoviridae
/
Adjuvants, Immunologic
/
Vaccines, DNA
/
HIV Envelope Protein gp160
/
Interleukin-15
/
Allergy and Immunology
/
Immunity, Humoral
/
Genetic Vectors
Limits:
Animals
/
Female
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2014
Type:
Article
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