Development of A synthetic positive control for the nucleic acid detection of mumps virus / 病毒学报
Chinese Journal of Virology
;
(6): 544-547, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-356669
ABSTRACT
To rapidly identify the cross-contamination problems caused by the positive control in the process of mumps virus nucleic acid detection, a new mumps virus RNA positive control was developed in this study. Using the same primers and reaction conditions, the cross-contamination problems caused by the positive control could be readily identified by comparing the fragments lengths of the PCR products between the positive control and the samples. This new RNA positive control of mumps virus can be widely used in the diagnosis and genotyping of mumps virus as a better laboratory quality control.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Reference Standards
/
Virology
/
RNA, Viral
/
Cell Line
/
DNA Primers
/
Reverse Transcriptase Polymerase Chain Reaction
/
Diagnosis
/
Genetics
/
Genotype
/
Methods
Type of study:
Diagnostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2013
Type:
Article
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