Establish A duplex fluorescent quantitative one-step RT-PCR system for the detection of norovirus genogroup I and II / 病毒学报
Chinese Journal of Virology
;
(6): 310-315, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-356686
ABSTRACT
The object of this study is to develop a duplex fluorescent quantitative one-step RT-PCR assay for detection and quantitation of GI and GII norovirus. The specific primers, Taqman probes, optimized reaction solution and condition were used to develop the duplex fluorescent quantitative one-step RT-PCR assay. The sensitivity, specificity and reproducibility of the assay were evaluated. The assay was evaluated by testing the 100 specimen samples and compared with the reference assay conventional RT-PCR. The assay possessed high specificity for norovirus detection without any evident cross-reaction with enteric adenovirus, rotavirus or astrovirus. The detection limit of the real-time RT-PCR assay, for GI and GII norovirus was up to 10(3) copy/microL respectively. Compared with the conventional RT-PCR assay, the assay in this study had higher sensitivity with higher detection rate of norovirus in stool specimens. The duplex fluorescent quantitative one-step RT-PCR assay provides rapid, sensitive and reliable detection of GI and GII norovirus, and could be used as a laboratory diagnosis of norovirus in acute gastroenteritis patients.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Virology
/
Classification
/
DNA Primers
/
Reverse Transcriptase Polymerase Chain Reaction
/
Norovirus
/
Diagnosis
/
Feces
/
Gastroenteritis
/
Genetics
/
Genotype
Type of study:
Diagnostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Virology
Year:
2013
Type:
Article
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