Determination of salvianolic acid B and paeoniflorin in Shuangdan granules by HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1062-1064, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-356700
ABSTRACT
<p><b>OBJECTIVE</b>To develop a method for the determination of salvianolic acid B and paeoniflorin in Shuangdan granules.</p><p><b>METHOD</b>The chromatographic separation was performed on a Zorbax SB C18 column with a linear gradient elution of phosphoric acid-water (0.05100) and phosphoric acid-acetonitrile-methanol (0.05496) was used. The flow rate was 1 mL x min(-1) and column temperature was set at 25 degrees C. The UV detection wavelength was set at 230 nm.</p><p><b>RESULT</b>The linear ranger was of 0.710-22.7 microg for salvianolic acid B and 0.103-3.30 microg for paeoniflorin. Three batches of Shuangdan granules were analyzed, and the content of salvianolic acid B ranged from 6.46-11.6 mg x g(-1) and paeoniflorin ranged from 1.44-1.78 mg x g(-1).</p><p><b>CONCLUSION</b>The method was accurate, reproducible, reliable, and could be used for quantitative control of Shuangdan granules.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plants, Medicinal
/
Quality Control
/
Benzoates
/
Benzofurans
/
Bridged-Ring Compounds
/
Drugs, Chinese Herbal
/
Chemistry
/
Reproducibility of Results
/
Chromatography, High Pressure Liquid
/
Salvia miltiorrhiza
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2006
Type:
Article
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