Study on quality for Herba Polygoni Orientalis / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish the qualitative and quantitative detective methods of Herba Polygoni Orientalis.</p><p><b>METHOD</b>Isorientin, orientin, protocatechuic acid and gallic acid in Herba Polygoni Orientalis were identified by TLC. The contents of isorientin and orientin in Herba Polygoni Orientalis were determined by HPLC.</p><p><b>RESULT</b>Isorientin, orientin, protocatechuic acid and gallic acid could be identified by TLC. Isorientin and orientin were well separated with Diamonsil C18 column and acetonitrile-0.1% phosphoric acid (1882) as mobile phase. The linear range of isorientin was 0.075-0.90 microg. The average recovery of isorientin was 98.8% and RSD was 2.1%. The linear range of orientin was 0.041-0.49 microg. The average recovery of orientin was 98.8% and RSD was 2.1%.</p><p><b>CONCLUSION</b>The methods can be used for qualitative identification and quantitation determination of Herba Polygoni Orientalis.</p>