Mechanism of Shenqi compound recipe anti-earlier diabetic artherosclerosis in GK rats / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1272-1276, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-356726
ABSTRACT
<p><b>OBJECTIVE</b>To explore the mechanism of Shenqi compound recipe (SQCR) anti-earlier diabetic artherosclerosis in GK rats.</p><p><b>METHOD</b>Four-month specefic pathogen free (SPF) GK rats were divided randomly according to blood glucose level into four groups model group (5 mL x kg(-1) x d(-1) sterile water), ramipril group (positive control, 1 mg x kg(-1) x d(-1)), SQCR low dosage (0.72 g x kg(-1) x d(-1)) and SQCR high dosage group (2.88 g x kg(-1) x d(-1)) and Wistar rats as normal control group(5 mL x kg(-1) x d(-1) sterile water). GK rats took high-fat diet freely and meanwile were injected N-omega-nitro-L-arginine methyl ester (L-N-AME) intra-peritoneally with the dose of 10 mg x kg(-1) x d(-1) in order to induce earlier diabetic artherosclerosis, while normal control group took regular diet and were injected normal saline intra-peritoneally. In the experiment periods, each group was administrated correspondent substance respectively for 32 d. At the end, sampling blood by abdominal aorta and picking aorta on ice. Determined monocyte chemoattractant protein-1 (MCP-1) concentration by ELISA, messenger ribonucleic acid (mRNA) expression of MCP-1 and peroxisome proliferator-activated receptor gamma (PPARgamma) in aorta by reverse transcriptase PCR (RT-PCR).</p><p><b>RESULT</b>Concentrations of MCP-1 in serum in SQCR low and high dosage groups and the mRNA expression of MCP-1 in SQCR high dosage group were all decreased significantly compared with model group (P < 0.05). The mRNA expression of PPARgamma in SQCR low and high dosage groups all increased compared with model group (P < 0.05 or P < 0.01).</p><p><b>CONCLUSION</b>Inhibiting the mRNA and protein expression of MCP-1 and upregulating the mRNA expression of PPARgamma in aorta might be contribute to SQCR anti-earlier diabetic artherosclerosis in GK rats partly.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Aorta
/
Pharmacology
/
Plants, Medicinal
/
Blood
/
RNA, Messenger
/
Drugs, Chinese Herbal
/
Random Allocation
/
Chemistry
/
Rats, Wistar
/
Chemokine CCL2
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2006
Type:
Article
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