Quantitative analysis of steroidal saponins in Chinese material medica Rhizoma Paridis by HPLC-ELSD / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>The several species of the genus Paris called as Rhizoma Paridis were famous traditional Chinese medica. To develop the quantitative analysis method of the steroidal saponins in some species of the genus Paris and commercially available Rhizoma Paridis samples by HPLC-ELSD.</p><p><b>METHOD</b>The contents of 11 steroidal saponins in Rhizoma Paridis samples were dectected with a Kromasil C18(4.6 mm x 150 mm, 5 microm) column which was deluted with acetonitrile-water (3070-6040) at a flow rate of 1 mL x min(-1) by HPLC-ELSD.</p><p><b>RESULT</b>All the authentic samples could be separated and calibration curves of 11 saponins were prepared. 11 steroidal saponins in 16 Rhizoma Paridis samples were detected in 30 min. The recovery for the assay of saponins was between 95% and 97%. The precision and stability of samples (RSD) were below 3%.</p><p><b>CONCLUSION</b>The method was shown to be accurate and convenient, and suitable for the quantitative analysis of these 11 steroidal saponins in the commercially available Rhizoma Paridis samples.</p>