Determnination of betaine in Fufang Guilu granule by HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 462-464, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-356794
ABSTRACT
<p><b>OBJECTIVE</b>To describe a HPLC method for assessing betaine in Fufang Guilu granule.</p><p><b>METHOD</b>The content of betainephenaxcyl bromide in Fufang Guilu granule was determined by HPLC. The analytical column was a shim-pack CLC-ODS (6.0 mm x 150 mm) filling a 5 microm stationary phase; The mobile phase consisted of acetonitrile-water(3565) with 0.1 mol x L(-1) NaClO4; The flow-rate was 1 mL x min (-1); The detector was set at 254 nm.</p><p><b>RESULT</b>The calibration curve was linear over the range of 0.09-0.585 microg (r = 0.9997). The average recovery of the method was 98.4%, RSD 2.5% (n = 5).</p><p><b>CONCLUSION</b>The results showed that this method was reliable and accurate, and can be used for quality control of Fufang Guilu granule.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plants, Medicinal
/
Quality Control
/
Betaine
/
Drugs, Chinese Herbal
/
Chemistry
/
Reproducibility of Results
/
Chromatography, High Pressure Liquid
/
Codonopsis
/
Lycium
/
Drug Combinations
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2006
Type:
Article
Similar
MEDLINE
...
LILACS
LIS