Cloning the full-length cDNA of a gene responsible for vascular smooth muscle cell proliferation in atherogenesis and study of function / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 166-170, 2003.
Article
in English
| WPRIM
| ID: wpr-356842
ABSTRACT
<p><b>OBJECTIVE</b>To clone the full-length cDNA of a gene responsible for vascular smooth muscle cell (v-SMC) proliferation in atherogenesis, and study its function.</p><p><b>METHODS</b>Oxidized low density lipoprotein (ox-LDL) at optimal concentration was used as the stimulant to induce v-SMC proliferation in culture medium. A cDNA subtractive library of v-SMC proliferation specific to ox-LDL stimulation was established using subtractive hybridization technique. Methods, including blotting, Northern hybridization and gene sequencing, were used to clone new gene fragments. By using full-length cDNA screening and protein expression techniques, one full-length cDNA was cloned and its function was studied.</p><p><b>RESULTS</b>One full-length cDNA was cloned. The new gene (Genbank AF 174647) expressed a 44 kDa protein, which might be associated with the activity of ox-LDL.</p><p><b>CONCLUSION</b>The new gene cloned may be associated with SMC proliferation in atherogenesis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Arteriosclerosis
/
Molecular Sequence Data
/
Base Sequence
/
Gene Library
/
Cell Division
/
Cells, Cultured
/
Blotting, Northern
/
Amino Acid Sequence
/
Cloning, Molecular
Limits:
Humans
Language:
English
Journal:
Chinese Medical Journal
Year:
2003
Type:
Article
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