Transfection of B7-1 cDNA empowers antigen presentation of blood malignant cells for activation of anti-tumor T cells / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 78-84, 2003.
Article
in English
| WPRIM
| ID: wpr-356864
ABSTRACT
<p><b>OBJECTIVE</b>To define roles of B7-1 co-stimulation factor expressed in human malignant cell lines in mediating anti-tumor T cell immune responses.</p><p><b>METHODS</b>Examining human leucocyte antigen (HLA) and B7 expressions on 8 human blood malignancies cell lines by flow cytometry. Transfecting B7-1 gene to B7-1 negative (B7(-)) Raji and B7(-) Jurkat cell lines by liposome, and comparing the potencies of blood malignant cell lines in the induction of T cell activation by examination of T cell cytokine mRNAs before and after transfection using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>High level of HLA I and II molecules were expressed in most human blood malignant cell lines examined, and the co-stimulatory factor B7-2 was also highly expressed. In contrast, another member of B7 family B7-1 was either not expressed or very limitedly expressed in most of these hematopoietic malignant cell lines. Most importantly, transfection of B7-1 gene to B7(-). Raji and B7(-). Jurkat cell lines made these cell lines better antigen presenting cells for stimulation of anti-tumor T cell activation, which was demonstrated by up regulation of expression of T cell cytokines IL-2, IL-4 and INF-gamma mRNAs after incubation of these tumor cells with T cells for 24 h.</p><p><b>CONCLUSIONS</b>B7 co-stimulation plays an important role in anti-tumor immunity. Transfection of B7-1 gene to the human hematopoietic malignant cell lines that are deficient in the B7-1 expression empowers their antigen presentation potency for activation of anti-tumor T cells. Our results suggested that repairing the deficiency of B7-1 co-stimulatory pathway in tumor cells might be a novel immunotherapeutic approach for human hematopoietic malignancies.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
RNA, Messenger
/
Membrane Glycoproteins
/
Lymphocyte Activation
/
T-Lymphocytes
/
Transfection
/
Antigens, CD
/
HLA-DR7 Antigen
/
Cytokines
/
B7-1 Antigen
Limits:
Humans
Language:
English
Journal:
Chinese Medical Journal
Year:
2003
Type:
Article
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