Genotype identification of Orientia tsutsugamushi isolated from Nan Peng Lie Islands in China / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 1881-1882, 2002.
Article
in English
| WPRIM
| ID: wpr-356887
ABSTRACT
<p><b>OBJECTIVE</b>To identify genotype of eight strains of Orientia tsutsugamushi (O. tsutsugamushi) isolated from Nan Peng Lie Islands in China and establish tsutsugamushi disease nature foci for this region.</p><p><b>METHODS</b>The nested polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) were used. Three primers were selected from the DNA sequence of the gene encoding type-specific 56-kDa protein of the Karp strain. The positive products were digested by Hine II and Pst I, meanwhile profiles specific to each strain were analyzed.</p><p><b>RESULTS</b>Three genotypes of O. tsutsugamushi including Karp, Kato and a new strain existed on Nan Peng Lie Islands.</p><p><b>CONCLUSION</b>Nan Peng Lie Islands is tsutsugamushi disease nature foci.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Orientia tsutsugamushi
/
Polymorphism, Restriction Fragment Length
/
DNA, Bacterial
/
China
/
Genetics
/
Genotype
Type of study:
Diagnostic study
Limits:
Animals
Country/Region as subject:
Asia
Language:
English
Journal:
Chinese Medical Journal
Year:
2002
Type:
Article
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