miR-221 mediates epithelial-mesenchymal transition-related gene expressions via regulation of PTEN/Akt signaling in drug-resistant glioma cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 218-222, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-356951
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the correlation between miR-221 and epithelial-mesenchymal transition (EMT) in drug-resistant glioma cells.</p><p><b>METHODS</b>The expression levels of miR-221, PTEN, p-Akt, E-cadherin, vimentin, and MRP1 were quantitatively analyzed in Z1 cells (primary drug-resistant cells), Z2 cells (drug-sensitive cells) and Z2-BCNU cells (drug-resistant cells) using fluorescent real-time PCR and Western blotting.</p><p><b>RESULTS</b>The expression levels of PTEN were significantly increased in Z2 cells compared with Z1 and Z2-BCNU cells which overexpressed miR-221 and vimentin. The expression levels of vimentin, p-Akt and MRP1 were significantly decreased in Z2 cells overexpressing E-cadherin.</p><p><b>CONCLUSION</b>MiR-221 regulates the expression of EMT-related genes through down-regulation of PTEN and activation of PI3-K/Akt signaling.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Vimentin
/
Signal Transduction
/
Cadherins
/
Gene Expression Regulation, Neoplastic
/
Drug Resistance, Neoplasm
/
Multidrug Resistance-Associated Proteins
/
MicroRNAs
/
Cell Line, Tumor
/
PTEN Phosphohydrolase
/
Proto-Oncogene Proteins c-akt
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2014
Type:
Article
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