The study of erythrocyte fragility and morphological changes caused by roller pump in vitro / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 419-422, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-357012
ABSTRACT
To observe the erythrocyte fragility and morphological changes of erythrocytes caused by roller pump. Ten tests were divided into two groups, Polystan pediatric pump group A (n = 5) and COBE pump group B (n = 5). Ten whole blood samples (each 400 ml) were circulated in the roller pump for 16 h. Erythrocyte fragility and free hemoglobin were measured before pumping and at every 2 hours during pumping. The possible morphological changes of erythrocytes caused by roller pump were observed by scanning electron microscope. The electron microscopic observation was made before pumping and at every 4 hours throughout pumping. Results showed that the erythrocyte fragility of two groups was not increased during a long period of pumping. The number of acanthocytes of two groups was 1.77/1.81% in the samples before pumping and 6.12/7.13, 9.18/8.73, 13.21/12.89, 16.53/17.21% at 4 h, 8 h, 12 h, and 16 h respectively. The free hemoglobin level of two groups was increased linearly during a long duration of pumping and the index of hemolysis of two groups was 0.296 mg/L/h and 0.3993 mg/L/h respectively. The result shows 1. the erythrocyte fragility was not increased during a long period of pumping; 2. the erythrocyte membrane was injured or broken by roller pump directly; 3. the morphological changes of erythrocytes would be the basis of post operative hemolysis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osmotic Fragility
/
Time Factors
/
In Vitro Techniques
/
Hemoglobins
/
Erythrocyte Deformability
/
Heart-Lung Machine
/
Hemolysis
Limits:
Humans
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2002
Type:
Article
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