Influence of the metabolite produced by Trichomonas vaginalis on human sperm motility in vitro / 中华男科学杂志
National Journal of Andrology
;
(12): 272-274, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-357030
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of the metabolite produced by Trichomonas vaginalis on human sperm motility in vitro.</p><p><b>METHODS</b>Trichomonas vaginalis having been cultured, the culture solution containing metabolite was obtained by removing the protozoa, then diluted into 3 kinds of concentration. Sperm was obtained from 10 healthy fertile men by masturbation and prepared by swim-up technique to produce a spermatozoon solution of high motility. Every sperm sample was divided into 4 groups (A, B, C, D). Unused culture solution was added to Group A as control, and the other 3 groups (B, C, D) were respectively incubated with the above used culture solution at 3 kinds of concentration (1.2 x 10(9)/L, 6 x 10(8)/L, 1.2 x 10(8)/L). Measurements were carried out at 30 s, 1 h, 2 h, 4 h, 6 h by CASA.</p><p><b>RESULTS</b>Sperm motility decreased in both Group B and C markedly, and the effects displayed a concentration- and time-dependent manner.</p><p><b>CONCLUSION</b>The metabolite of Trichomonas vaginalis can reduce human sperm motility in vitro, and may be one of the causes of infertility.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Sperm Motility
/
Time Factors
/
Trichomonas vaginalis
/
Dose-Response Relationship, Drug
/
Metabolism
Limits:
Animals
/
Humans
/
Male
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2004
Type:
Article
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