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Fusion expression of Neisseria gonorrhoeae outmembrane protein with a mucosal adjuvant / 中华男科学杂志
National Journal of Andrology ; (12): 269-274, 2004.
Article in Chinese | WPRIM | ID: wpr-357031
ABSTRACT
<p><b>OBJECTIVE</b>To express a fusion protein of Neisseria gonorrhoeae with a mucosal adjuvant.</p><p><b>METHODS</b>The gene coding Loop VI-VIII(PL678) of porin, an out-membrane protein of Neisseria gonorrhoeae, was obtained by PCR. It was inserted into a plasmid fused with subunit B of heat labile enterotoxin. The recombinant was transformed in E. coli. The expression of fusion protein was analysed by ELISA, SDS-PAGE and Western-blot.</p><p><b>RESULT</b>Fusion protein with LTB was successfully expressed, and displayed both the ability of binding GM1 and the reactogenicity with polyclonal antibodies against Neisseria gonorrhoeae.</p><p><b>CONCLUSION</b>The expression of fusion protein laid a foundation for the study of the intramolecular vaccine against Neisseria gonorrhoeae.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Bacterial Outer Membrane Proteins / Bacterial Toxins / Recombinant Fusion Proteins / Bacterial Vaccines / Vaccines, Synthetic / Chemistry / Polymerase Chain Reaction / Escherichia coli Proteins / Enterotoxins / Allergy and Immunology Language: Chinese Journal: National Journal of Andrology Year: 2004 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Bacterial Outer Membrane Proteins / Bacterial Toxins / Recombinant Fusion Proteins / Bacterial Vaccines / Vaccines, Synthetic / Chemistry / Polymerase Chain Reaction / Escherichia coli Proteins / Enterotoxins / Allergy and Immunology Language: Chinese Journal: National Journal of Andrology Year: 2004 Type: Article