Protective effect of nitric oxide synthase inhibitor (L-NAME) on germ cell apoptosis in experimentally cryptorchid rats / 中华男科学杂志
National Journal of Andrology
;
(12): 684-689, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-357107
ABSTRACT
<p><b>OBJECTIVE</b>To explore the protective effect of nitric oxide synthase inhibitor (L-NAME) on the germ cell apoptosis in the rat cryptorchid.</p><p><b>METHODS</b>Immature rats (22 day-old Sprague Dawley) were subjected to unilateral cryptorchid. Thirty rats were divided into three groups sham operation group (testes still in the scrotum after operation); operation group; operation + L-NAME group(given L-NAME 10 mg/kg after operation, dip). Seven days after operation germ cell apoptosis was detected by terminal-deoxynucleotidyl transferase mediated-dUTP nick end labeling(TUNEL). Biochemical parameters (NO, NOS) were evaluated with spectrophotometric determination.</p><p><b>RESULTS</b>At the 7th day after the operation, compared with the control, the number of apoptotic germ cells in the cryptorchid testis was increased significantly, but the testis weight was decreased predominantly(P < 0.01). The levels of NO and NOS in the cryptorchid were significantly higher than the control.</p><p><b>CONCLUSIONS</b>The levels of NO and NOS might be involved in the germ cell apoptosis in the cryptorchid; L-NAME could protect the germ cell from apoptosis in experimentally cryptorchid rats by reducing the activity of NOS and reducing the level of NO in the testis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Physiology
/
Spermatozoa
/
Rats, Sprague-Dawley
/
Apoptosis
/
Nitric Oxide Synthase
/
NG-Nitroarginine Methyl Ester
/
Protective Agents
/
Cryptorchidism
Limits:
Animals
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2003
Type:
Article
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