Effect of As₂O₃on Hedgehog Pathway in Chronic Myeloid Leukemia Cells / 中国实验血液学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To explore the effect of As₂O₃on Hedgehog pathway in chronic myeloid leukemia (CML) cells.</p><p><b>METHODS</b>The apoptosis of K562 cells was detected by MTT method and flow cytometry; the expressions of PTCH and SMO protein and mRNA in Hedgehog pathway were determined by Western blot and real-time PCR, respectively. Retults The As₂O₃ could induce the apoptosis of K562 cells with optimal concentration 2 µmol/L and optimal time 24 hours. The expressions of PTCH and SMO protein and mRNA in Hedgehog pathway of K562 cells treated with As₂O₃at optimal concentration and optimal time were down-regulated.</p><p><b>CONCLUSION</b>The As₂O₃can down-regulate the expression of PTCH and SMO in Hedgehog pathway.</p>