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Effect of SAHA on Maturation of Dendritic Cells and Its Mechanism / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 821-825, 2015.
Article in Chinese | WPRIM | ID: wpr-357265
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of SAHA on the maturation of human dendritic cells (DC) and to explore its underlying mechanism.</p><p><b>METHODS</b>Peripheral blood mononuclear cells (PBMNC) were isolated from human peripheral blood and cultured in RPMI 1640 medium with 100 ng/ml rhGM-CSF and 500 U/ml rhIL-4. In the LPS induced maturation process, dendritic cells treated with or without SAHA were used as test group, and dendritic cells treated without LPS or SAHA were used as control group. DC was observed under inverted microscope. Flow cytometer was used to detect the surface antigen molecules expressed by DC. The mixed lymphocyte culture (MLC) was used to observe the allogeneic lymphocyte stimulation. The NF-κB signaling pathway was detected by electrophoretic mobility shift assay (EMSA).</p><p><b>RESULTS</b>The SAHA could effectively suppress the maturation of DC induced by LPS, the DC treated with SAHA+LPS had immature morphological characteristics; the expression of CD80, CD83 and HLA-DR in SAHA+LPS group and control group were significantly down-regulated as compared with single LPS group (P<0.01); the ability of DC to stimulate the proliferation of allogeneic T lymphocytes in SAHA+LPS group and control group was significantly weaker than that in single LPS group (P<0.01); EMSA results showed that NF-κB activity decreased after SAHA and LPS treatment and was significantly lower than that of single LPS group.</p><p><b>CONCLUSION</b>SAHA can effectively suppress DC maturation induced by LPS and also weaken the ability to stimulate allogeneic T lymphocyte. NF-κB signaling pathway is involved in regulating DC maturation.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Dendritic Cells / Lymphocyte Activation / T-Lymphocytes / HLA-DR Antigens / Cell Differentiation / NF-kappa B / Lymphocyte Culture Test, Mixed / Flow Cytometry Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2015 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Dendritic Cells / Lymphocyte Activation / T-Lymphocytes / HLA-DR Antigens / Cell Differentiation / NF-kappa B / Lymphocyte Culture Test, Mixed / Flow Cytometry Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2015 Type: Article