Follicular Helper T Cells and Expression of PD-1 in Mice with Myelodysplastic Syndrome / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 756-760, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-357276
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the complete blood count, morphological changes, follicular T helper (Tfh) cells and expression of PD-1 in bone marrow and spleen of mice with myelodysplastic syndrome(MDS) and to explore their significance in pathogenesis of MDS.</p><p><b>METHODS</b>The 10 male NUP98-HOXD13 transgenic mice and 10 male homologous wild-type C57BL/6J mice were used for experments. The complete blood count, morphological change of NUP98-HOXD13 transgenic mice and wild-type C57BL/6J were detected by routine methods. The level of Tfh cells and expression of PD-1 in bone marrow and spleen were measured by flow cytometry. The PD-1 mRNA of bone marrow mononuclear cells and spleen cells were analyzed by real-time PCR method.</p><p><b>RESULTS</b>The counts of RBC, neutrophile and platelet in above- mentioned transgenic mice were less than that in wild type C57BL/6J mice. As compared with wild type C57BL/6J mice, the morphology of RBC and platelet in transgenic mice was some abnormal, including bi-nucleated erythrocytes, ringed mucleated neutrophil and erythroblastic islands. The count of Tfh cells in transgenic mice was less than that in wild type mice, but the expression of PD-1 was higher. The expression of BMMNC PD-1 mRNA was obviously higher than that in wild type mice.</p><p><b>CONCLUSION</b>The pancytopenia and dysplasia, decrease of Tfh cells and increase of PD-1 expression have been observed in NUP98-HOXD13 transgenic mice, which may be one of important reasons for promoting malignant clone and leading to impair anti immune respones.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pancytopenia
/
Bone Marrow
/
Myelodysplastic Syndromes
/
Bone Marrow Cells
/
Mice, Transgenic
/
Cells, Cultured
/
T-Lymphocytes, Helper-Inducer
/
Programmed Cell Death 1 Receptor
/
Real-Time Polymerase Chain Reaction
/
Flow Cytometry
Limits:
Animals
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2015
Type:
Article
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