Salinomycin Enhances the Apoptosis of T-cell Acute Lymphoblastic Leukemia Cell Line Jurkat Cells Induced by Vincristine / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 653-657, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-357297
ABSTRACT
<p><b>OBJECTIVE</b>This study was aimed to investigate the effect of salinomycin combined with vincristine on the proliferation and apoptosis of Jurkat cells and its possible mechanisms.</p><p><b>METHODS</b>The proliferation of Jurkat cells was examined by CKK-8 assay. Flow cytometry was used to assess cellular apoptosis. Levels of BCL-2, caspase-3, and caspase- 8 were measured by Western blot.</p><p><b>RESULTS</b>The salinomycin or vincristine, either alone or in combination, inhibited the proliferation of Jurkat cells in a dose-dependent manner. Salinomycin combined with vincristine produced more obveous inhibition of cell proliferation than either compound used alone (P<0.05). Western blot analysis showed that the combined use of Sal and VCR reduced the expression of BCL-2 protein, and increased expression of caspase 3 and caspase 8 protein, more significantly. Furthermore, combination of Sal and VCR synergistally promoted apoptosis of the Jurkat cells (P<0.05).</p><p><b>CONCLUSION</b>The combination of salinomycin and vincristine synergistically inhibits proliferation and promotes apoptosis of T-cell acute lymphoblastic leukemia Jurkat cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pyrans
/
Vincristine
/
Apoptosis
/
Jurkat Cells
/
Cell Proliferation
/
Caspase 3
/
Caspase 8
/
Precursor T-Cell Lymphoblastic Leukemia-Lymphoma
/
Flow Cytometry
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2015
Type:
Article
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