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Mannan-Binding Lectin Inhibits Candida Albicans-Induced DC Maturation and Cytokine Secretion / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 517-521, 2015.
Article in Chinese | WPRIM | ID: wpr-357324
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of mannan-binding lectin (MBL) on the maturation and cytokine secretion of human dendritic cells (DC) induced by Candida albicans (C. albicans).</p><p><b>METHODS</b>The plastic-adherent mononuclear cells were prepared from the blood of healthy adult volunteers. The human peripheral blood mononuclear cells-derived dendritic cells (MNC-DC) were induced by 5-day-culture in medium supplemented with rhGM-CSF and rhIL-4, and then cultured for 2 days in presence or absence of C. albicans at varying concentration of human MBL ranging from 1 to 20 mg/L. DC's shape and characters were observed under inverted microscopy, the expression of CD83 and CD86 on DC was analyzed by FACS. The levels of TNF-α and IL-6 were detected by ELISA. FACS also was used to investigate the interaction of MBL with immature DC(imDC) and C. albicans. Western blot was used to detect C. albicans-induced IκBα phosphorylation and p65/NF-κB translocation in DC.</p><p><b>RESULTS</b>MBL at higher concentrations (10-20 mg/L) down-regulated the expression of CD83 and CD86 on the monocyte-derived dentritic cells(MoDC) induced by C. albicans, and inhibited the production of TNF-α and IL-6 induced by C. albicans. FACS showed that MBL could not only bind to C. albicans but also bind to imDCs in a Ca2+-dependent manner. Western blot showed that MBL could decrease the phosphorylation of IκBα and the nuclear translocation of p65/ NF-κB.</p><p><b>CONCLUSION</b>MBL may inhibit TNF-α and IL-6 production induced by C. albicans in DC through NF-κB signaling pathways, suggesting that MBL can play some roles in the regulation of C. albicans-induced immune response.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Dendritic Cells / Candida albicans / Cell Differentiation / Cytokines / NF-kappa B / Protein Transport / Mannose-Binding Lectin Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2015 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Dendritic Cells / Candida albicans / Cell Differentiation / Cytokines / NF-kappa B / Protein Transport / Mannose-Binding Lectin Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2015 Type: Article