Effects of PBDE-47 on oxidative stress and apoptosis in SH-SY5Y cell / 中华劳动卫生职业病杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the oxidative stress and apoptosis induced by 2, 2', 4, 4'-polybrominated diphenyl ethers (PBDE-47) in human neuroblastoma SH-SY5Y cells, and to explore the involved role of reactive oxygen species (ROS) on apoptosis.</p><p><b>METHODS</b>The rate of cellular survivors, intracellular ROS level, malondialdehyde (MDA) content and percentage of apoptosis were measured respectively after the SH-SY5Y cell were exposed to 2, 4, 8 microg/ml PBDE-47 for 24 h in vitro.</p><p><b>RESULTS</b>The rate of cellular survivors in the low dose PBDE-47-treated group was higher than the control group (P < 0.05), but the moderate and high dose PBDE-47-treated groups were significantly lower than the control group (P < 0.05). The MDA contents in the moderate and high dose PBDE-47-treated groups were significantly higher than the control group (P < 0.05) and increased with increase of PBDE-47 exposure concentrations. Compared with the control group, the levels of ROS were significantly increased with increase of PBDE-47 concentrations (P < 0.05). In the moderate and high dose PBDE-47-treated groups, the percentages of apoptosis were significantly higher than that of the control group (P < 0.05).</p><p><b>CONCLUSION</b>PBDE-47 can induce oxidative stress and apoptosis in SH-SY5Y cell. ROS may play an important role in the apoptosis induced by PBDE-47.</p>