Effect of human silicotic alveolar macrophages on the expression of the collagen type I in human embryonic lung fibroblasts / 中华劳动卫生职业病杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the effect of the cultured supernatant of human silicotic alveolar macrophages (AM) on the expression of the collagen type I in human embryonic lung fibroblasts.</p><p><b>METHODS</b>Human alveolar macrophages were collected from a silicotic patient by bronchoalveolar lavage and exposed to silicon dioxide for 18 h. Then the cultured supernatant were used to culture human embryonic lung fibroblasts for 6 h, 12 h, 18 h, 24 h, 36 h, 48 h, 72 h. Then detected collagen anabolism and secretion with (3)H-proline detected the expression of the procollagen type I in the fibroblast with immunological method detected the quantity of collagen Type I in FB supernatant with Western blot.</p><p><b>RESULTS</b>The anabolism and secretion of collagen were increased in cultured supernatant of silicotic AM exposed to SiO(2), Along with the time, the expression of collagen type I increased. In cultured supernatant of silicotic AM exposed to SiO(2), ((3)H-proline 1096.500 +/- 76.400, 707.000 +/- 62.160, OD 0.314 +/- 0.011, OD 14.218 +/- 0.342.</p><p><b>CONCLUSION</b>SiO(2) may affect the expression of collagen through AM mediation and participate in the formation of lung fibrosis.</p>