ALA-PDT alleviates graft-versus-host disease in mice following allogenic bone marrow transplantation / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 408-412, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-357803
ABSTRACT
<p><b>OBJECTIVE</b>This study investigated the hypothesis that 5-aminolevulinic acid-mediated photodynamic therapy (ALA-PDT) might alleviate acute graft-versus-host disease (GVHD) following allogenic bone marrow transplantation (allo-BMT) in mice.</p><p><b>METHODS</b>Acute GVHD model following allo-BMT was established in 40 recipient BALB/C mice. Fifty C57BL/6J mice were used as donors and another 10 BALB/C mice as blank control without any intervention. Recipients received a lethal dose of 8.5 Gy (60)Co radiation for 10 minutes before transplantation and then were randomly divided into four groups of 10 mice (A-D). Group A was injected with normal saline injection and served as controls. Group B received pure donor bone marrow and spleen cell infusion. Group C received donor bone marrow and mixed donor-recipient spleen cell infusion. Group D was administered with an infusion of donor bone marrow cells and mixed donor-recipient spleen cells treated with ALA-PDT. The 28th day survival rate, incidence of acute GVHD and hematological and pathological changes after transplantation were examined.</p><p><b>RESULTS</b>All the mice from the Blank control group survived. The survival rates for Groups A-D on the 28th day were 0, 0, 10% and 60% respectively. Group D showed a significantly higher survival rate than the other three groups (P < 0.01). Most of the mice in Groups B and C developed GVHD but only two developed in Group D. Moreover Group D had less severe hematological and pathological changes when compared with Groups B and C.</p><p><b>CONCLUSIONS</b>ALA-PDT significantly alleviated GVHD and increased the 28th day survival rate for allo-BMT mice. ALA-PDT may be a promising therapy for GVHD following allo-BMT. Future studies should focus on the underlying mechanism of its therapeutic effect.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Photochemotherapy
/
Transplantation, Homologous
/
Mortality
/
Bone Marrow Transplantation
/
Therapeutic Uses
/
Drug Therapy
/
Graft vs Host Disease
/
Aminolevulinic Acid
/
Leukocyte Count
/
Mice, Inbred BALB C
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Contemporary Pediatrics
Year:
2006
Type:
Article
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