Expression of G-6-PD mRNA in children with G-6-PD deficiency / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 385-387, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-357808
ABSTRACT
<p><b>OBJECTIVE</b>To detect the level of glucose-6-phosphate dehydrogenase (G-6-PD) mRNA expression in children with G-6-PD deficiency and their lineal family members in order to explore possible mechanisms of the disease at the transcriptional level.</p><p><b>METHODS</b>RNA was extracted from peripheral blood of 41 children with G-6-PD deficiency and of their lineal family members (29 father lineages and 40 mother lineages). cDNA was then harvested using the reverse transcription method. G-6-PD mRNA expression was detected by quantitative real-time PCR (QRT-PCR). The detection results of G-6-PD mRNA expression in three groups (Patient, Father lineage and Mother lineage) were compared using Statistical Software SPSS 10.0 system.</p><p><b>RESULTS</b>The mean G-6-PD mRNA value in the Patient, Father lineage and Mother lineage groups were 0.57 +/- 0.19, 0.74 +/- 0.21 and 0.67 +/- 0.21 respectively. The G-6-PD mRNA value in the Patient group was significantly lower than both Father lineage (t = -3.18, P < 0.01) and Mother lineage groups (t = -2.54, P < 0.05).</p><p><b>CONCLUSIONS</b>The expression of G-6-PD mRNA decreased in children with G-6-PD deficiency, suggesting that the pathogenesis of this disorder relates to the varying levels of gene transcription.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
RNA, Messenger
/
Reverse Transcriptase Polymerase Chain Reaction
/
Genetics
/
Glucosephosphate Dehydrogenase
/
Glucosephosphate Dehydrogenase Deficiency
Limits:
Child
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Contemporary Pediatrics
Year:
2006
Type:
Article
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