Study on extraction of coumarins in Cnidium monieri by supercritical CO2 and separation of compositions analysis / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To analyze the chemical constituents of supercritical carbon dioxide extraction products from Cnidium monieri.</p><p><b>METHOD</b>Four-factor and three-level orthogonal experimental design was used to optimize the SFE conditions as guided by the content of total coumarins in the extract. The chemical constituents were separated and identified by recrystalization.</p><p><b>RESULT</b>Optimum extraction process was established 25 MPa as extraction pressure, 50 degrees C as extraction temperature, 6.5 MPa as separation pressure and 60 degrees C as separation temperature.</p><p><b>CONCLUSION</b>Changes in extraction pressure, temperature, time, pulverized degree and separation pressure affect the extracting results remarkably. The two kinds of chemical constituents were separated by recrystallization from C. monieri and identified by the methods of UV, IR, MS, NMR.</p>