ITS sequence identification of Radix Bupleuri / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 732-734, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-358123
ABSTRACT
<p><b>OBJECTIVE</b>To provide the basis of molecular authentication of Radix Bupleuri by the comparison of the internal transcribed spacer (ITS) sequences of five kinds of Radix Bupleuri in common use.</p><p><b>METHOD</b>Firstly, total DNA of five kinds of Radix Bupleuri was extracted. Secondly, the ITS sequence was amplified by PCR with universal primer of ITS and PCR product was directly sequenced after purification.</p><p><b>RESULT</b>The length of ITS1 and ITS2 sequence was 214-220 bp and 230-231 bp respectively. There were distinct variation sites between the ITS sequences of the five kinds of Radix Bupleuri.</p><p><b>CONCLUSION</b>ITS sequence may be the evidence for the molecular authentication of Radix Bupleuri.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phylogeny
/
Plants, Medicinal
/
Molecular Sequence Data
/
Base Sequence
/
Classification
/
Plant Roots
/
DNA, Plant
/
DNA, Ribosomal Spacer
/
Bupleurum
/
Cuminum
Type of study:
Diagnostic study
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2005
Type:
Article
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