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Bacterial entombment by intratubular mineralization following orthograde mineral trioxide aggregate obturation: a scanning electron microscopy study / 国际口腔科学杂志·英文版
International Journal of Oral Science ; (4): 227-232, 2014.
Article in English | WPRIM | ID: wpr-358133
ABSTRACT
The time domain entombment of bacteria by intratubular mineralization following orthograde canal obturation with mineral trioxide aggregate (MTA) was studied by scanning electron microscopy (SEM). Single-rooted human premolars (n=60) were instrumented to an apical size #50/0.06 using ProFile and treated as follows Group 1 (n=10) was filled with phosphate buffered saline (PBS); Group 2 (n=10) was incubated with Enterococcus faecalis for 3 weeks, and then filled with PBS; Group 3 (n=20) was obturated orthograde with a paste of OrthoMTA (BioMTA, Seoul, Korea) and PBS; and Group 4 (n=20) was incubated with E. faecalis for 3 weeks and then obturated with OrthoMTA-PBS paste. Following their treatments, the coronal openings were sealed with PBS-soaked cotton and intermediate restorative material (IRM), and the roots were then stored in PBS for 1, 2, 4, 8 or 16 weeks. After each incubation period, the roots were split and their dentin/MTA interfaces examined in both longitudinal and horizontal directions by SEM. There appeared to be an increase in intratubular mineralization over time in the OrthoMTA-filled roots (Groups 3 and 4). Furthermore, there was a gradual entombment of bacteria within the dentinal tubules in the E. faecalis inoculated MTA-filled roots (Group 4). Therefore, the orthograde obturation of root canals with OrthoMTA mixed with PBS may create a favorable environment for bacterial entombment by intratubular mineralization.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Oxides / Physiology / Root Canal Filling Materials / Root Canal Obturation / Time Factors / Zinc Oxide-Eugenol Cement / Calcification, Physiologic / Microscopy, Electron, Scanning / Enterococcus faecalis / Silicates Limits: Humans Language: English Journal: International Journal of Oral Science Year: 2014 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Oxides / Physiology / Root Canal Filling Materials / Root Canal Obturation / Time Factors / Zinc Oxide-Eugenol Cement / Calcification, Physiologic / Microscopy, Electron, Scanning / Enterococcus faecalis / Silicates Limits: Humans Language: English Journal: International Journal of Oral Science Year: 2014 Type: Article