Identification of the involvement of LOXL4 in generation of keratocystic odontogenic tumors by RNA-Seq analysis / 国际口腔科学杂志·英文版
International Journal of Oral Science
;
(4): 31-38, 2014.
Article
in English
| WPRIM
| ID: wpr-358154
ABSTRACT
Keratocystic odontogenic tumors (KCOT) are benign, locally aggressive intraosseous tumors of odontogenic origin. KCOT have a higher stromal microvessel density (MVD) than dentigerous cysts (DC) and normal oral mucosa. To identify genes in the stroma of KCOT involved in tumor development and progression, RNA sequencing (RNA-Seq) was performed using samples from KCOT and primary stromal fibroblasts isolated from gingival tissues. Seven candidate genes that possess a function potentially related to KCOT progression were selected and their expression levels were confirmed by quantitative PCR, immunohistochemistry and enzyme-linked immunosorbent assay. Expression of lysyl oxidase-like 4 (LOXL4), the only candidate gene that encodes a secreted protein, was enhanced at both the mRNA and protein levels in KCOT stromal tissues and primary KCOT stromal fibroblasts compared to control tissues and primary fibroblasts (P<0.05). In vitro, high expression of LOXL4 could enhance proliferation and migration of the human umbilical vein endothelial cells (HUVECs). There was a significant, positive correlation between LOXL4 protein expression and MVD in stroma of KCOT and control tissues (r=0.882). These data suggest that abnormal expression of LOXL4 of KCOT may enhance angiogenesis in KCOT, which may help to promote the locally aggressive biological behavior of KCOT.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Dentigerous Cyst
/
Odontogenic Tumors
/
Gene Expression Regulation, Enzymologic
/
Cell Movement
/
Stromal Cells
/
Sequence Analysis, RNA
/
Disease Progression
/
Cell Proliferation
/
Microvessels
Type of study:
Diagnostic study
Limits:
Adult
/
Female
/
Humans
/
Male
Language:
English
Journal:
International Journal of Oral Science
Year:
2014
Type:
Article
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