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Inhibition of matrix metalloproteinases expression in human dental pulp cells by all-trans retinoic acid / 国际口腔科学杂志·英文版
International Journal of Oral Science ; (4): 150-153, 2014.
Article in English | WPRIM | ID: wpr-358170
ABSTRACT
All-trans retinoic acid (ATRA) inhibits matrix metalloproteinase (MMP)-2 and MMP-9 in synovial fibroblasts, skin fibroblasts, bronchoalveolar lavage cells and cancer cells, but activates MMP-9 in neuroblast and leukemia cells. Very little is known regarding whether ATRA can activate or inhibit MMPs in human dental pulp cells (HDPCs). The purpose of this study was to determine the effects of ATRA on the production and secretion of MMP-2 and -9 in HDPCs. The productions and messenger RNA (mRNA) expressions of MMP-2 and -9 were accessed by gelatin zymography and real-time polymerase chain reaction (PCR), respectively. ATRA was found to decrease MMP-2 level in a dose-dependent manner. Significant reduction in MMP-2 mRNA expression was also observed in HDPCs treated with 25 µmol⋅L(-1) ATRA. However, HDPCs treated with ATRA had no effect on the pattern of MMP-9 produced or secreted in either cell extracts or conditioned medium fractions. Taken together, ATRA had an inhibitory effect on MMP-2 expression in HDPCs, which suggests that ATRA could be a candidate as a medicament which could control the inflammation of pulp tissue in vital pulp therapy and regenerative endodontics.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Transcription, Genetic / Tretinoin / RNA, Messenger / Cell Survival / Cells, Cultured / Culture Media, Conditioned / Cell Culture Techniques / Matrix Metalloproteinase 2 / Matrix Metalloproteinase 9 Limits: Humans Language: English Journal: International Journal of Oral Science Year: 2014 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Transcription, Genetic / Tretinoin / RNA, Messenger / Cell Survival / Cells, Cultured / Culture Media, Conditioned / Cell Culture Techniques / Matrix Metalloproteinase 2 / Matrix Metalloproteinase 9 Limits: Humans Language: English Journal: International Journal of Oral Science Year: 2014 Type: Article