Regulatory role of Syk gene on vascular endothelial growth factor C expression in breast cancer / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 805-809, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-358230
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of Syk on the VEGF-C expression in breast cancer.</p><p><b>METHODS</b>Immunohistochemical EnVision method was used to detect the protein expression of Syk, NFκB and VEGF-C in breast carcinoma; and the relationship between protein expression of Syk, NFκB, VEGF-C and lymph node metastasis was analysed. MDA-MB-231 cells were transfected with pcDNA3.1(-)-Syk, and the effect of Syk gene on the VEGF-C and NFκB expression was determined.</p><p><b>RESULTS</b>In the lymph node metastatic group, a lower expression rate of Syk and higher expression rate of VEGF-C and NFκB were detected as compared to the non-metastatic group. The expression of Syk was negatively associated with NFκB (r = -0.448, P = 0.002) and VEGF-C (r = -0.620, P = 0.000) expression, and VEGF-C was associated with the nuclear expression of NFκB (r = 0.310, P = 0.036). Compared with the non-transfected cells, the pcDNA3.1(-)-Syk transfected MDA-MB-231 cells showed significantly lower transcriptional level of VEGF-C mRNA, expression level of VEGF-C protein and NFκB activity (P < 0.05).</p><p><b>CONCLUSIONS</b>Syk may play an important role in the lymph node metastasis of breast cancer. It may down-regulate the expression of VEGF-C by inhibiting the activity of NFκB, which thus suppresses lymph node metastasis of breast cancer.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Physiology
/
Plasmids
/
Protein-Tyrosine Kinases
/
Breast Neoplasms
/
RNA, Messenger
/
Transfection
/
NF-kappa B
/
Carcinoma, Medullary
/
Carcinoma, Lobular
Limits:
Adult
/
Aged
/
Female
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Pathology
Year:
2011
Type:
Article
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