Apoptosis-inducing effect of carbazole alkaloid (HY-1) in human erythroleukemia K562 cells / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 457-460, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-358604
ABSTRACT
<p><b>OBJECTIVE</b>To investigate apoptosis-inducing effect and its mechanisms of HY-1, a carbazole alkaloid, on human erythroleukemia K562 cells.</p><p><b>METHODS</b>Cell proliferation was detected by sulforhodamine B (SRB) assay after treated with HY-1 at indicated doses. Cell cycle analysis was performed by flow cytometry, mitochondria membrane voltage change was assessed by rhodamine 123 staining, annexin V-PI apoptosis detecting kit and DNA agarose gel electrophoresis were used to identify apoptosis-inducing effect of HY-1. The alterations of apoptosis-relating proteins were detected by Western blot.</p><p><b>RESULTS</b>The IC(50) of HY-1 in K562 cells was (29.05 +/- 0.90) micromol/L by SRB assay. HY-1 had significant apoptotic inducing effect on K562 cells in a dose- and time-dependent manner as verified by appearance of Sub-G(1) peak on histogram of flow cytometry analysis, reduction of mitochondria membrane voltage, appearance of double positive cell group in Annexin V-PI apoptosis detecting test, and remarkable DNA ladder. The expression of cytosolic cytochrome c was apparently increased. Pro-caspase-9, pro-caspase-3 and PARP were all cleaved to active segments. There was no change in the expression of caspase-8.</p><p><b>CONCLUSION</b>HY-1 exerts its anticancer activity through triggering apoptosis of K562 cells by mitochondria-activating pathways.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Carbazoles
/
Chemistry
/
Apoptosis
/
K562 Cells
/
Rutaceae
/
Alkaloids
/
Metabolism
/
Mitochondria
/
Antineoplastic Agents, Phytogenic
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Oncology
Year:
2005
Type:
Article
Similar
MEDLINE
...
LILACS
LIS