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Screening and identification of phage-displayed polypeptides specifically binding to human gastric cancer with high metastatic potential to peritoneum / 中华肿瘤杂志
Chinese Journal of Oncology ; (12): 397-400, 2005.
Article in Chinese | WPRIM | ID: wpr-358622
ABSTRACT
<p><b>OBJECTIVE</b>By means of phage-display technique, to screen polypeptides that specifically bind to human gastric cancer with high metastatic potential to peritoneum.</p><p><b>METHODS</b>Two human gastric cancer cell lines were used GC9811-P with high metastatic potential to peritoneum and its wild type parental GC9811, to carry out subtractive screening with a phage display-12 peptide library.</p><p><b>RESULTS</b>After three rounds of screening, 40 phage clones bond to GC9811-P cells were randomly selected. When injected into the peritoneal cavity of nude mice, 6 of the 40 clones did not bind to mouse peritoneum as examined by immunohistochemical staining. They were considered to be capable of binding specifically to GC9811-P cells. Sequence analysis revealed two different exogenous peptides TLNINRLILPRT and SMSI(X)SPYI(XXX).</p><p><b>CONCLUSION</b>Two peptides have been obtained that specifically bind to a gastric cancer cell variant GC9811-P, which easily disseminates to the peritoneum. Whether or not they could block GC9811-P metastasis to peritoneum in vivo remains to be determined.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Peptides / Peritoneal Neoplasms / Protein Binding / Stomach Neoplasms / Binding Sites / Enzyme-Linked Immunosorbent Assay / Sensitivity and Specificity / Peptide Library / Protein Array Analysis Type of study: Diagnostic study / Screening study Limits: Animals / Female / Humans / Male Language: Chinese Journal: Chinese Journal of Oncology Year: 2005 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Peptides / Peritoneal Neoplasms / Protein Binding / Stomach Neoplasms / Binding Sites / Enzyme-Linked Immunosorbent Assay / Sensitivity and Specificity / Peptide Library / Protein Array Analysis Type of study: Diagnostic study / Screening study Limits: Animals / Female / Humans / Male Language: Chinese Journal: Chinese Journal of Oncology Year: 2005 Type: Article