The effect of niflumic acid on gamma aminobutyric acid activated current in DRG neurons / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 68-71, 2013.
Article
in Zh
| WPRIM
| ID: wpr-358676
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the modulatory effect of niflumic acid (NFA) on gamma aminobutyric acid (GABA)-activated currents of dorsal root ganglion (DRG) neurons in rat.</p><p><b>METHODS</b>The whole-cell patch-clamp technique was used to record the NFA- and GABA-activated currents in neurons freshly dissociated from rat DRG neurons.</p><p><b>RESULTS</b>Application of NFA(0.1 - 100 micromol/L) could induce concentration-dependent outward currents in some cells (21/48,43.75%), and GABA (0.1 - 100 micromol/L) could induce concentration-dependent inward currents in some cells(150/159,94.32%). NFA-(100 micromol/L) and GABA-(100 micromol/L) activated currents were (0.27 +/- 0.06) nA (n = 12) and (1.29 +/- 0.72) nA (n = 53) respectively. However, pre-application of NFA (0.1 - 100 micromol/L) could inhibit the GABA-activated inward current which was identified to be GABAA receptor-mediated current. The inhibitory effects of NFA were concentration-dependent. NFA could not alter the EC50 (about 30 micromol/L) and inverse potential (about -10 mV) of GABA-activated current (P > 0.05).</p><p><b>CONCLUSION</b>Pre-application of NFA exerts a more strong inhibitory effect on the peak value of GABA-activated current.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Physiology
/
Cell Separation
/
Cells, Cultured
/
Niflumic Acid
/
Rats, Sprague-Dawley
/
Patch-Clamp Techniques
/
Gamma-Aminobutyric Acid
/
Ganglia, Spinal
/
Metabolism
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Applied Physiology
Year:
2013
Type:
Article