Preliminary research of induction of the multiple HPV antibody by HPV L1 type conserved sequence aimed at human papillomavirus major protein / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 982-987, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-359140
ABSTRACT
To investigate whether a conserved sequence of the human papillomavirus(HPV) L1 protein consisted of 12 amino acid residue can induce the antibody aimed at multiple HPV types, we screened a conserved sequence of the HPV L1 protein by forecasting B cell epitope and comparing multiple sequences. The peptide was synthesized, mixed with Freund adjuvant, and used to immunize rabbits, and those in the control group were only immunized with Freund adjuvant. Then the antibody titer was identified by indirect enzyme-linked immunosorbent assay (ELISA). And immunocytochemistry, immunofluorescence, western blot and immunohistochemistry were used to detect whether the antibody could react with cervical cancer cell lines and cervical tissue that had been identified with HPV infections. We found that the antibody titer was greater than 125600. Moreover, we confirmed that the antibody could react with cervical cancer cell lines and cervical tissue with HPV infections. The results showed that the peptide could induce antibody aimed at multiple HPV types. Our findings have great significance in further research of the broad spectrum HPV, HPV L1 diagnosis kits.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Papillomaviridae
/
Viral Proteins
/
Computer Simulation
/
B-Lymphocytes
/
HeLa Cells
/
Oncogene Proteins, Viral
/
Classification
/
Papillomavirus Infections
/
Capsid Proteins
/
Allergy and Immunology
Type of study:
Prognostic study
Limits:
Animals
/
Female
/
Humans
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2011
Type:
Article
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