Rolipram, a Phosphodiesterase 4 Inhibitor, Stimulates Inducible cAMP Early Repressor Expression in Osteoblasts
Yonsei Medical Journal
;
: 149-154, 2005.
Article
in English
| WPRIM
| ID: wpr-35920
ABSTRACT
Phosphodiesterase (PDE) 4 inhibitors have been shown to induce the cAMP-mediated signaling pathway by inhibiting cAMP hydrolysis. This study investigated the effect of a PDE4 inhibitor on the expression of the inducible cAMP early repressor (ICER), which is an endogenous inhibitor of CRE- mediated transcription, in osteoblastic cells. RT-PCR analysis revealed that rolipram, a PDE4 inhibitor, stimulates the ICER mRNA in a dose dependent manner. The induction of ICER mRNA expression by rolipram was suppressed by the inhibitors of protein kinase A (PKA) and p38 MAPK, suggesting the involvement of PKA and p38 MAPK activation in ICER expression by rolipram. It was previously shown that rolipram induced the expression of TNF-related activation-induced cytokine (TRANCE, also known as RANKL, ODF, or OPGL) in osteoblasts. This paper provides evidences that a transcriptional repressor like ICER might modulate TRANCE mRNA expression by rolipram in osteoblasts.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteoblasts
/
Phosphodiesterase Inhibitors
/
Transcription Factors
/
Gene Expression
/
Cyclic AMP-Dependent Protein Kinases
/
Rolipram
/
Animals, Outbred Strains
/
P38 Mitogen-Activated Protein Kinases
/
DNA-Binding Proteins
Limits:
Animals
Language:
English
Journal:
Yonsei Medical Journal
Year:
2005
Type:
Article
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