Albumin resuscitation protects against traumatic/hemorrhagic shock-induced lung apoptosis in rats / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B
;
(12): 871-878, 2008.
Article
in English
| WPRIM
| ID: wpr-359347
ABSTRACT
<p><b>OBJECTIVE</b>To determine the effects of albumin administration on lung injury and apoptosis in traumatic/hemorrhagic shock (T/HS) rats.</p><p><b>METHODS</b>Studies were performed on an in vivo model of spontaneously breathing rats with induced T/HS; the rats were subjected to femur fracture, ischemia for 30 min, and reperfusion for 20 min with Ringer's lactate solution (RS) or 5% (w/v) albumin (ALB), and the left lower lobes of the lungs were resected.</p><p><b>RESULTS</b>Albumin administered during reperfusion markedly attenuated injury of the lung and decreased the concentration of lactic acid and the number of in situ TdT-mediated dUTP nick-end labelling (TUNEL)-positive cells. Moreover, immunohistochemistry performed 24 h after reperfusion revealed increases in the level of nuclear factor kappaB (NF-kappaB), and phosphorylated p38 mitogen-activated protein kinase (MAPK) in the albumin-untreated group was down-regulated by albumin treatment when compared with the sham rats.</p><p><b>CONCLUSION</b>Resuscitation with albumin attenuates tissue injury and inhibits T/HS-induced apoptosis in the lung via the p38 MAPK signal transduction pathway that functions to stimulate the activation of NF-kappaB.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oxygen
/
Pathology
/
Resuscitation
/
Shock, Hemorrhagic
/
Blood
/
Immunohistochemistry
/
Reperfusion Injury
/
Random Allocation
/
Rats, Sprague-Dawley
/
Apoptosis
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Journal of Zhejiang University. Science. B
Year:
2008
Type:
Article
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