Induction of nucleoside phosphorylase in Enterobacter aerogenes and enzymatic synthesis of adenine arabinoside / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B
;
(12): 520-526, 2008.
Article
in English
| WPRIM
| ID: wpr-359396
ABSTRACT
Nucleoside phosphorylases (NPases) were found to be induced in Enterobacter aerogenes DGO-04, and cytidine and cytidine 5'-monophosphate (CMP) were the best inducers. Five mmol/L to fifteen mmol/L cytidine or CMP could distinctly increase the activities of purine nucleoside phosphorylase (PNPase), uridine phosphorylase (UPase) and thymidine phosphorylase (TPase) when they were added into medium from 0 to 8 h. In the process of enzymatic synthesis of adenine arabinoside from adenine and uracil arabinoside with wet cells of Enterobacter aerogenes DGO-04 induced by cytidine or CMP, the reaction time could be shortened from 36 to 6 h. After enzymatic reaction the activity of NPase in the cells induced remained higher than that in the cells uninduced.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pentosyltransferases
/
Pharmacology
/
Vidarabine
/
Enzyme Induction
/
Enterobacter aerogenes
/
Cytidine
/
Cytidine Monophosphate
Language:
English
Journal:
Journal of Zhejiang University. Science. B
Year:
2008
Type:
Article
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