Using oligonucleotide suspension arrays for laboratory identification of bacteria responsible for bacteremia / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B
;
(12): 291-298, 2008.
Article
in English
| WPRIM
| ID: wpr-359430
ABSTRACT
The aim of this study was to develop and validate an oligonucleotide suspension array for rapid identification of 15 bacterial species responsible for bacteremia, particularly prevalent in Chinese hospitals. The multiplexed array, based on the QIAGEN LiquiChip Workstation, included 15 oligonucleotide probes which were covalently bound to different bead sets. PCR amplicons of a variable region of the bacterial 23S rRNA genes were hybridized to the bead-bound probes. Thirty-eight strains belonging to 15 species were correctly identified on the basis of their corresponding species-specific hybridization profiles. The results show that the suspension array, in a single assay, can differentiate isolates over a wide range of strains and species, and suggest the potential utility of suspension array system to clinical laboratory diagnosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oligonucleotides
/
Stem Cells
/
RNA, Ribosomal
/
RNA, Ribosomal, 23S
/
DNA Probes
/
Chemistry
/
Bacteriological Techniques
/
Genetic Techniques
/
Bacterial Typing Techniques
/
Bacteremia
Type of study:
Diagnostic study
/
Prognostic study
Language:
English
Journal:
Journal of Zhejiang University. Science. B
Year:
2008
Type:
Article
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