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Development of Silk Fibroin Composite Films for Corneal Repair / 生物医学工程学杂志
Journal of Biomedical Engineering ; (6): 867-886, 2015.
Article in Chinese | WPRIM | ID: wpr-359555
ABSTRACT
It is important to design a long-period transparent bioactive material for corneal repair in the process of corneal tissue renovation. This article discusses the silk fibroin and formamide blend membranes as a corneal stroma repair material. Silk fibroin solution was mixed with formamide in different proportions to obtain insoluble transparent silk fibroin film by casting method. The blending membranes had excellent mechanical properties, cell compatibility and long-term transparent properties. Rabbit corneal stromal cells were seeded on the sterilized composite films. The rate of cell surface adhesion was over 90% after cells were placed on it for 5 hours. When cells were seeded on blend membranes from one day to seven days, Alma Blue was added to complete medium. Compared with the cell culture plate, there was no significant difference in cell proliferation on formamide/silk films. The results indicated that formamide/silk films might be used as a corneal stroma repair material and worth of further investigatinn
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Regeneration / Biocompatible Materials / Cell Adhesion / Chemistry / Cornea / Cell Biology / Cell Proliferation / Fibroins Limits: Animals Language: Chinese Journal: Journal of Biomedical Engineering Year: 2015 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Regeneration / Biocompatible Materials / Cell Adhesion / Chemistry / Cornea / Cell Biology / Cell Proliferation / Fibroins Limits: Animals Language: Chinese Journal: Journal of Biomedical Engineering Year: 2015 Type: Article