Quantitative Analysis of Immuno-fluorescence of Nuclear Factor-κB Activation / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 669-674, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-359588
ABSTRACT
Immuno-fluorescence technique can qualitatively determine certain nuclear translocation, of which NF-κB/ p65 implicates the activation of NF-κB signal pathways. Immuno-fluorescence analysis software with independent property rights is able to quantitatively analyze dynamic location of NF-κB/p65 by computing relative fluorescence units in nuclei and cytoplasm. We verified the quantitative analysis by Western Blot. When we applied the software to analysis of nuclear translocation in lipopolysaccharide (LPS) induced (0. 5 h, 1 h, 2 h, 4 h) primary human umbilical vein endothelial cells (HUVECs) , we found that nuclear translocation peak showed up at 2h as with calculated Western blot verification results, indicating that the inventive immuno-fluorescence analysis software can be applied to the quantitative analysis of immuno-fluorescence.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Software
/
Cell Nucleus
/
Fluorescent Antibody Technique
/
Active Transport, Cell Nucleus
/
Cytoplasm
/
NF-kappa B p50 Subunit
/
Human Umbilical Vein Endothelial Cells
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2015
Type:
Article
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