Assessment Method of Remnant α-1, 3-galactosyle Epitopes in Animal Tissue-derived Biomaterials / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 662-679, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-359589
ABSTRACT
The aim of this study was to establish an assessment method for determining α-Gal (α-1, 3-galactosyle) epitopes contained in animal tissue or animal tissue-derived biological materials with ELISA inhibition assay. Firstly, a 96 well plate was coated with Gal α-1, 3-Gal/bovine serum albumin (BSA) as a solid phase antigen and meanwhile, the anti-α-Gal M86 was used to react with α-Gal antigens which contained in the test materials. Then, the residual antibodies (M86) in the supernatant of M86-Gal reaction mixture were measured using ELISA inhibition assay by the α-Gal coating plate. The inhibition curve of the ELISA inhibition assay, the R2 = 0.999, was well established. Checking using both α-Gal positive materials (rat liver tissues) and α-Gal negative materials (human placenta tissues) showed a good sensitivity and specificity. Based on the presently established method, the α-Gal expression profile of rat tissues, decellular animal tissue-derived biological materials and porcine dermal before and after decellular treatment were determined. The M86 ELISA inhibition assay method, which can quantitatively determine the α-Gal antigens contained in animal tissues or animal tissue-derived biomaterials, was refined. This M86 specific antibody based-ELISA inhibition assay established in the present study has good sensitivity and specificity, and could be a useful method for determining remnant α-1, 3Gal antigens in animal tissue-derived biomaterials.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Trisaccharides
/
Biocompatible Materials
/
Serum Albumin, Bovine
/
Enzyme-Linked Immunosorbent Assay
/
Sensitivity and Specificity
/
Methods
/
Antibodies
/
Epitopes
Type of study:
Diagnostic study
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2015
Type:
Article
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