Development of an identification method for Streptococcus oligofermentans: a new species of oral streptococci with molecular markers / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 712-715, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-359634
ABSTRACT
<p><b>OBJECTIVE</b>To establish a quick and reliable method to identify Streptococcus oligofermentans, a new species of oral streptococci.</p><p><b>METHODS</b>With two-step PCR, a pair of the 16S rDNA-specific primers of Streptococcus oligofermentans and a pair of primers of lactate oxidase gene (lox) were used to amplify the gene fragments from the genomic DNAs of 11 strains consisting of 9 species of the pure culture of oral streptococci. Pooled plaque samples from 9 caries-free volunteers were cultured on a selective medium of MSA with erythromycin and tentative strains of Streptococcus oligofermentans were isolated. The isolates were further identified by the two-step PCR and finally confirmed by 16S rDNA sequence analysis.</p><p><b>RESULTS</b>With the two-step PCR, the two gene fragments were only amplified from the three identified strains of Streptococcus oligofermentans, but not the rest of 8 strains of oral streptococci. Isolates from the dental plaque of caries-free volunteers were identified as Streptococcus oligofermentans by PCR and then further confirmed by 16S rDNA sequence homology analysis.</p><p><b>CONCLUSIONS</b>Streptococcus oligofermentans could be identified by the two-step PCR approach with the specific 16S rDNA primers and lactate oxidase gene primers.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Streptococcus
/
DNA, Bacterial
/
DNA, Ribosomal
/
RNA, Ribosomal, 16S
/
Polymerase Chain Reaction
/
Bacterial Typing Techniques
/
Classification
/
Sequence Analysis, DNA
/
DNA Primers
/
Genetics
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Chinese Journal of Stomatology
Year:
2007
Type:
Article
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